Abstract: Objective：To explore the long-term efficacy of specific subcutaneous immunotherapy (SCIT) with standardized house dust mite on children with asthma and allergic rhinitis. Methods：This paper was a case series report for long-term follow-up of intervention effects. Children with house dust mite allergic asthma and allergic rhinitis received SCIT (T0) to a course of 30 or more months of treatment (T1)，at T0, T1, T2 (3 years after T1) and T3 (6 years after T1), either in asthma specialist out-patient or by telephone follow-up. Many indexes were assessed, including asthma symptom scores (ASS), rhinitis symptom score (RSS), total symptom score (TSS), total medication score (TMS), symptom medication score (SMS). Visual analogue scale (VAS) and improvement self-evaluation were also used. ASS, RSS, TSS and TMS were evaluated for nearly 4 weeks. VAS and the condition improved self-evaluation for the children or their parents were used to assess the timing of their own feelings for nearly 1 month. Results：From April 2006 to June 2011, 56 cases ( 41 male cases, average age 7.1 (5~12) years old) were diagnosed as dust mite allergic asthma and allergic rhinitis children in the Asthma specialist outpatient department, and received SCIT treatment ≥ 30 months, meanwhile ASS, RSS, TSS and VAS at T0 were collected retrospectively, and TSS and SMS were calculated. There were 51 cases (38 male cases) at T2 and 45 cases (33 male cases) at T3. All evaluation indexes at T1 were significantly different compared with those at T0. There were significant differences in the 2 composite indexes (TSS and SMS), which reflected the assessment of the illness between any two adjacent time points. In the improvement self-evaluation, the differences of allergic rhinitis and asthma at T1 (18.5% vs 75%), T2 (31.2% vs 84%), T3 (39.5% vs 80%) were statistically significant. In the condition of improving the self-assessment scale，the condition to evaluate allergic rhinitis is obviously worse than asthma. At T3 point, the RSS, TSS, SMS of girls are significantly lower than those of boys and the difference is statistically significant (P<0.05). Conclusion：For children with dust mite allergic asthma and allergic rhinitis, SCIT can improve the asthma and rhinitis symptom markedly, decrease medication and VAS score. The long-term effect can maintain for 6 years after the cessation of treatment. The RSS, TSS and SMS of female children were more effective than those of male children.