Abstract: Objective To conduct a systematic review and meta-analysis of the literature on central line-associated bloodstream infections (CLABSI) in children based on a standardized central line bundle so as to provide the best evidence for the prevention and control of CLABSI in children. Methods Literature that met all of the following criteria was included: a. Populations were NICU and PICU patients younger than 18 years old; b. A standardized central line bundle was used as the intervention which referred to hand hygiene, maximal sterile barrier precautions, skin disinfectants of >0.5% chlorhexidine, iodine or iodophor, daily audits to assess whether each central line was still needed and ly removal of unnecessary central lines and the best insertion site (except femoral vein) based on individual patient characteristics and the first four of the above requirements about the bundle should be met at least; c. The outcome was the number of CLABSI events within 1,000 catheter days; d. The literature belonged to studies of interventions; e. There were no limits to the language of literature. Literature was searched in the database of Ovid-MEDLINE, Ovid-EMBASE, Web of science, Cochrane Library and China Biology Medicine (CBM), CNKI, VIP and Wanfang from January 1st, 2003 to December 31st, 2019. The risk of bias for eligible studies was assessed according to Risk Of Bias In Non-randomized Studies of Interventions (ROBINS-I). Results A total of 9,708 literature was retrieved including 2 articles from other sources. Forty-one studies from 20 countries were included with 30 for NICU,10 for PICU and 1 for both, 18 of which were historical control studies from America with standardized central line bundles as the post-intervention and no limits to pre-interventions. There was a total of 952,584 catheter days, including 442,296 for pre-interventions and 510,288 for the post-interventions. Among 41 included literature, studies with low, moderate, serious risk of bias accounted for 27% (11 studies), 44% (18 studies) and 29%(12 studies) respectively. The standardized central line bundle could reduce the number of CLABSI events in both NICU and PICU patients［I 2=81%, random effect model, RR=0.40 (95% CI: 0.33-0.48), absolute effect value: 3 fewer per 1,000 catheter days (from 4 fewer to 3 fewer)］. For the intervention site of NICU, the number of CLABSI events in neonates can be reduced ［I 2=85%, random effect model, RR=0.36 (95% CI: 0.29-0.45), absolute effect value: 3 fewer per 1,000 catheter days (from 4 fewer to 3 fewer)］. For the intervention site of PICU, the number of CLABSI events in children can be reduced ［I 2=27%, fixed effect model, RR=0.54 (95% CI: 0.42-0.69), absolute effect value: 4 fewer per 1,000 catheter days (from 5 fewer to 3 fewer)］. Conclusion Standardized central line bundles (at least including hand hygiene, maximal sterile barrier precautions, skin disinfectants of >0.5% chlorhexidine, iodine or iodophor and daily audits and ly removal of unnecessary central lines) for pediatric patients could reduce 3 to 4 cases of CLABSI per 1,000 catheter days.