Abstract:

Objective To study the protective effects of CORM3 treatment on AECs apoptosis after LPS inducing acute lung injury in neonatal rats. Methods Thirty two SD neonate rats were divided equally into four groups, the control group, LPS group,CORM3 group and iCORM3 group. Neonate rat acute lung injury was induced by LPS intratracheal administration in LPS group, CORM3 group and iCORM3 group received treatment of itraperitoneal injection of saline, CORM3 and iCORM3 respectively. The control group received intraperitoneal injection of saline. Animals in each group were sacrificed after 12h modeling, the histopathologic changes were observed by HE staining, and lung tissue was seperated and weighed, wet and dry ratio of lung tissue was calculated, lung tissue damage was detected by BALF cell counting and protein quantitative analysis. Cultivated A549 cell apoptosis was induced by LPS in vitro, cell activity was determined by MTT test, cell apoptosis was watched by Tunel dyeing. Results Firstly, compared with the control group, the morphological structure of lung tissue was disordered in model group, alveoli were compressed, and a lot of inflammatory cells in filtrated in pulmonary interstitium. CORM3 group kept basic normal morphological structure, and inflammatory cells infiltrated in alveolar interstitium were less, the iCORM3 group was consistent with the LPS group in lung morphological structure and inflammatory cells infiltration. Secondly, compared with the control group, the W/D ratio, BALF cell number and protein content increased significantly in LPS group, and the number of AECs apoptosis was increased obviously［(37.3±4.5)% vs (3.0±1.0)%］. The A549 cells activity was decreased, and percentage of apoptosis cells increased significantly［(29.6±4.1)% vs (3.6±1.0)%, P<0.05］,with a statistically significant difference. In CORM3 group compared with the LPS group, the W/D ratio, BALF cell number and protein content decreased obviously, and the number of AECs apoptosis was decreased［（19.3±4.6）%］. The A549 cells activity rebounded, and the rate of cells apoptosis［（15.3±4.5）%］ decreased significantly, the difference was statistically significant(P < 0.05).There were no significant changes between the iCORM3 group and the CORM3 group in these indexes. Conclusion CORM3 attenuates neonate rat acute lung injury induced by LPS via inhibiting AECs apoptosis.