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Objective To analyze the differential item functioning (DIF) of Fine Motor Function Measure (FMFM) between hemiplegic and non-hemiplegic cerebral palsy. Methods Data of children with cerebral palsy diagnosed by Rehabilitation Center of Children's Hospital of Fudan University and Children Rehabilitation Collaboration Network of Children's Hospital from 2001 to 2018 were collected and assessed by FMFM as well. The contralateral data of hemiplegic children were extracted from FMFM evaluation data, and 30% samples were randomly selected as focus group samples of DIF. According to the proportion of non-hemiplegic type and hemiplegic type in the whole sample, randomized stratified matching was used to match the control group sample. Conquest software was used to make Rasch analysis of the two groups of merged data samples and all 56 items in B to E area of FMFM, and single-handed items and double-handed items were separated by analysis item functioning. The single dimensionality of the scale was determined by the mean square of cohesive fit (Infit), the standard was MnSq 0.6-1.4, and differential item functioning of all 56 items and both single and double-handed items were analyzed. Results Tetally 3,442 times of FMFM assessments of 1,556 children with cerebral palsy were taken as whole samples and data. One hundred and ninety-eight FMFM assessments of focus group and 792 FMFM assessments of control group were included in this study. Single sample pairing test showed that there was no significant difference in FMFM scores between the two groups [112 (96, 146) vs 113 (95, 145)]. After three turns of item functioning analysis, 30 were classified as single-handed items and 26 as double-handed items. The absolute value >0.5 of the difference between the numerical of difficulty scale pair of total 56 items is 41. The absolute value >0.5 of the difference between the numerical of difficulty scale pair of 30 single-handed and 26 double-handed items are 23 (76.7%) and 9 (34.6%) respectively. Conclusion There exists DIF between hemiplegic and non-hemiplegic cerebral palsy in all 56 items of FMFM scale, especially in single-handed items. In order to accurately evaluate the fine motor function of hemiplegic children, it is necessary to re-establish the evaluation criteria and procedures.

Objective To evaluate the applied meaning of Ig/TCR gene rearrangements by RQ-PCR for minimal residual disease(MRD) detection in childhood acute lymphoblastic leukemia （ALL）. Methods ①PCR detection of Ig/TCR gene rearrangements of ALL children was performed at diagnosis. ②The clonality of Ig/TCR gene rearrangements was analyzed by GeneScan. ③The sequence of monoclonal PCR products was checked to determine the patient-specific sequence as MRD detection target followed by the design of patient-specific primers and probes, and RQ-PCR quantification at different therapy phases. Results Eighty six children diagnosed as ALL were included. ①At least one type of Ig/TCR rearrangement was found in 96.5% (83/86) ALL children. ②91.8% (56/61) ALL children were detected to have at least one monoclonal Ig/TCR gene rearrangement. The detection rates for monoclonal, oligoclonal and polyclonal Ig/TCR rearrangement were 58.1%, 30.8% and 11.0% with significant difference(P<0.001). ③For 22 continuous complete remission(CCR) cases, the Ig/TCR gene rearrangement expression consistently decreased as therapy continued, and before maintaining therapy, all tested MRD cases were detected to be MRD negative; For 4 relapse cases, MRD was persistently positive from beginning of induced remission to relapse, and clinically the average relapse time was 3.75 months (ranging from 2 to 8 months) since the relative expression of Ig/TCR rearrangement started increasing. Conclusions Surveillance of ALL children's MRD level detected by RQ-PCR with Ig/TCR gene rearrangement as targets, is a potentially useful assay for evaluation and assessment of therapy effect and relapse prediction.

The Appraisal of Guidelines for Research & Evaluation (AGREE) instrument has been the important standard for Clinical Practice Guideline appraisal, since released in 2003. The second version, AGREEⅡ was released in 2009. Some domestic researchers has translated and used the instruments. We want to explain each items of AGREE Ⅱ detailedly through appraising Chinese Clinical Practice Guidelines, hoping to provide reference for the domestic researchers understanding and using AGREEⅡ appropriately.

Background: Sitting height and leg length are the useful indicators for the linear growth evaluation of children and adolescents, but the growth reference standards of the two indicators have not been reported. Objective: To study and formulate the growth reference of sitting height and leg length of Chinese children and adolescents aged 0-18 years. Design: A cross-sectional survey. Methods: Stature and sitting height of 92,494 healthy urban children aged 0-18 years were measured in two national surveys in 2005 in China—The National Survey on the Physical Growth and Development of Children in the Nine Cities of China (NSPGDC) and The Chinese National Survey on Students' Constitution and Health (CNSSCH). Leg length was calculated by height minus sitting height. Growth reference standards of sitting height and leg length were constructed using the LMS method. Main outcome measures: Reference values of sitting height and leg length. Results: The percentile and the Z-scores reference values of sitting height and leg length for Chinese boys and girls aged 0-18 years were shown and their growth charts were drawn. Sitting height increased about 14 cm, 6 cm and 4 cm during the first, second and third year after birth, respectively. After that, the annual increment of sitting height is nearly 2-3 cm. Then the increments became slightly higher at 11-13 years for boys and 9-11 years for girls and gradually became smaller and smaller. Sitting height nearly stopped increasing at the age of 17 years for boys and 15 years for girls. Leg length increased respectively about 11-12 cm and 6.5 cm during the first and second year after birth, then the annual increment was 4-5 cm during 2-5 years, and leg length stopped increasing nearly at 15 years for boys and 13 years for girls. Conclusion: The growth reference standards of sitting height and leg length were formulated, which provide more data and reference for perfecting the growth evaluation system of children and adolescents in China.

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Objective To explore the causal association between birth weight and childhood obesity by using the GWAS data and the Mendelian randomization (MR) method.Methods Based on the genetic data published by the Early Growth Genetics Consortium (EGG) (data acquisition time is October 20, 2018), the SNP with statistically significant association with birth weight was screened by MR-base platform. As instrumental variables (screening parameters P<5×10 ^-8, linkage disequilibrium r²<0.1), birth weight and childhood obesity were determined by inverse variance weighted analysis (IVW), weighted median method and MR-Egger method, respectively (definition of childhood obesity: BMI≥95%). Forest maps and scatter plots were also applied.Results A total of 33 SNPs related to birth weight were screened. A total of 5,530 obese children and 8,318 normal children were included. IVW analysis: OR=1.79, 95% CI: 1.29~2.47, P=4.24×10 ^-4; weighted median method: OR=1.30, 95% CI: 0.81~2.08, P=0.27; MR-Egger method: OR=1.73, 95% CI: 0.58~5.20, P=0.36. The result of IVW in the forest map showed that the genetic susceptibility of childhood obesity was related to the birth weight level. rs7964361, rs11765649, rs3780573 and rs1351394 showed that birth weight and childhood obesity were statistically significant. The scatter plot showed that the causal correlation estimates for the IVW method, the MR-Egger method, and the weighted median method were similar.Conclusion IVW analysis showed that there was a strong correlation between birth weight and childhood obesity, but the other two statistical methods found that the difference was not statistically significant, so the causal association remained to be supported by further evidence.

Background: Great heterogeneity exists in studies on the relationship between adolescents' mental health and 24-h movement consisting of sleep, sedentary behavior, and physical activity. Therefore, it is difficult to obtain high-quality evidence synthesis sufficient to guide clinical practice. Objective: To summarize and evaluate the current status of research on the relationship between 24-hour movement (sleep, sedentary behavior, and physical activity) and mental health in children and adolescents, as well as the problems in research design, so as to provide a reference for future research. Design: Scoping review. Methods: PubMed, Embase, Web of Science, and CNKI databases were searched from the inception to September 28, 2022, with the keywords of sleep, sedentary behavior, physical activity, 24-h movement, mental health, and adolescents to identify research on the correlation or causal inference between 24-h movement and mental health in healthy children and adolescents. Data extraction forms for movement type, measurement methods, classification of measurement items, and specific item measurement were gradually refined in the process of data extraction and synthesis after reading the text title, abstract, and full text for screening. Main outcome measures: Study types and measurement instruments for the relationship between 24-h movement and mental health in adolescents. Results: A total of 927 articles were retrieved from the English and Chinese databases, and after de-duplication, initial screening and full-text screening, 55 articles were finally included with 45 (82%) cross-sectional studies and 10 prospective cohort studies; 47 (85%) subjective reports and 8 objective monitoring articles. For sleep, 54 described sleep duration and 4 assessed sleep quality, sleep latency, daytime sleepiness, and sleep disruption. For sedentary behavior, there were 50 subjective reports, 5 objective reports, and 46 studies about screen exposure. For physical activity, hours of moderate-to-vigorous physical activity were assessed in 47 subjective reports and 8 objective reports. A total of 32 studies focused on depression, 9 on anxiety, 5 on stress, and 35 on other mental health outcomes (positive mood, behavioral problems, life satisfaction or health-related quality of life, mental illness and symptoms, emotion regulation skills, and cell phone addiction). Forty-four papers explored the correlation between 24-h movement and mental health, 8 papers explored changes in the structure of 24-h movement using isochronous substitution models, and 3 explored mediators of 24-h movement affecting mental health. Conclusions: There are few studies on the association between 24-h movement and mental health in adolescents, and the measurement tools used need to be standardized. In the future, isochronous substitution models or real-world intervention studies are needed to find the recommended 24-h movement packages that can maximize the health effects of the movement and are easy to achieve at the same time.

Objective: To investigate the consistency between the Greulich-Pyle method and the China 05 method for assessing bone age in normal children, and to compare the assessment results with the chronological age. Methods: All subjects, collected from 5 primary and secondary schools in Xuhui and Jing'an districts of Shanghai, were taken with orthotopic left-handed DR digital radiography, and two experts performed the GP method and the China 05 method, respectively. Pearson's analysis was used to analyze the correlation of the two methods and Kappa analysis was used to test the consistency of differences between the assessment results and the chronological age. Results: A total of 4,151 students, including 2,184 males and 1,967 females, completed the bone age sampling from December 2015 to October 2016. Pearson's analysis showed two assessment results were highly correlated in general and by developmental stages(r=0.888-0.988, P<0.000 1). The correlation coefficient r between the two methods and the chronological age ranged from 0.487 to 0.851 (P<0.001 for both). Kappa analysis showed the Kappa values were below 0.5 for both boys and girls in general and by age. The GP method values were less than that of the China 05 method (P<0.000 1) for males before the age of 10 and females before the age of 9. Assessment results of the two methods were basically the same for females at the age of 9 (P> 0.05) and the GP method values were greater than that of the China 05 method (P< 0.000 1) for males and females after the age of 9. The China 05 results were closer to the chronological age for both sexes. Conclusion: The consistency between the two methods of bone age assessment is low for normal children and adolescents, and the assessment of maturity status should be combined with other growth indicators. In this sample, the China 05 method was slightly better than the GP method.

Background：Chinese Infectious Disease Surveillance of Pediatrics(ISPED)was established in 2015. The data of antibiotics resistance were collected from each hospital and summarized every year to monitor the infectious etiology changes and bacterial drug resistance among Chinese children. Objective：This study is aimed to investigate the bacterial infectious and antimicrobial resistance profiles of pathogens in Chinese children and guide the reasonable use of antibiotics. Design：A crosssectional survey. Methods：Clinical isolates were collected from 13 tertiary children hospitals in China from January 1st to December 31st in 2021. Antimicrobial susceptibility testing was carried out according to a unified protocol using KirbyBauer method or automated systems. Penicillin susceptibility of streptococcus pneumonia was detected by Etest. All of the antimicrobial susceptibility testing results were interpreted according to the criteria of Clinical and Laboratory Standards Institute (CLSI) 2021. All members used the standard methods to perform bacterial culture,identification and antimicrobial susceptibility test and reported the isolated effective strains and bacterial resistance data in a structured format every year for data summary and statistical analysis. Main outcome measures：Detect the distribution of bacteria strains in children and the changes of bacteria drug resistance. Results：A total of 63 508 isolates were collected, of which 39.9% was grampositive organisms and 60.1% was gramnegative organisms. Top ten pathogens were as follows, Escherichia coli (12.7%), Streptococcus pneumonia (12.3%), Straphylococcus aureus (11.6%), Haemophilus influenza (11.4%), Moraxella catarrhalis (6.5%), Klebsiella pneunoniae (5.2%), Pseudomonas aeruginosa (4.0%), Staphylococcus epidermidis (3.7%), Acinetobacter baumannii (2.4%) and Streptococcus pyogenes (2.1%). The distribution of the primary pathogenic bacteria in different age groups was different. Among them, Saureus had the highest isolation rate in neonates (18.7%), and S.pneumoniae was the main pathogenic bacteria in children aged from >28 d to 5 years old, arranging from 13.6% to 20.8%. E.coli was the most common pathogen in children over 5 years old (22.4%). The proportion of penicillininsensitive S.pneumoniae (PNSP) decreased to 9.6%. Multidrugresistant bacteria (MDROs) showed high levels of resistance to a variety of clinical antibiotics, and the ratios of carbapenemresistant Enterobacteriaceae (CRE), K.pneumoniae (CRKP), P.aeruginosa (CRPA), A.baumannii (CRAB) were 4.6%, 14.8%, 6.7% and 30.7%, respectively. The proportions of CRPA and CRAB showed a decreasing trend in the past 6 years. The former had a higher detection rate in the neonatal group, while the latter had a higher detection rate in the nonneonatal group. Moreover, the resistance rates of CRPA and CRAB to multiple antibiotics were significantly higher in the nonneonatal group than in the neonatal group. Methicillinresistant Saureus(MRSA) was relatively stable, with a detection rate of 33.2% in 2021. Conclusion：The detection rate of MDROs in 2021 was lower than previous years, and the resistance ratio of MDROs also decreased in pediatric patients. We must pay attention to the infection and colonization of CRPA in the neonatal population.

Background：Rehabilitation of children and adolescents with cerebral palsy（CP） needs highquality clinical practice guidelines to provide effective assessment and intervention to improve the health condition of children and adolescents with CP. Objective：To improve and perfect the topic structure of Clinical Practice Guidelines for Children and Adolescents with Cerebral Palsy (CANDLE). Design：A Scoping review Methods：We searched English literature in Google Scholar and Chinese literature in CNKI, WangFang and VIP from January 1, 2010 to May 2, 2022 to get literature about children and adolescents with CP including guidelines, expert consensus, systematic review, and scoping review. Grey literature was searched in Google and BAIDU. According to intelligent sorting of Google scholar, researchers preliminarily screened literature by reading titles and abstracts until consecutive 50 articles of failed to match the inclusion criteria. The included literature was rescreened by reading full texts. References of the included literature after the second screening were compared with the final list of the included studies to find differences. Those unduplicated ones were screened by reading titles and abstracts. Researchers extracted data about questions proposed by the scoping review to a selfmade Excel sheet. The sheet consists of items of the application condition of The International Classification of Functioning, Disability and Health for Children and Youth（ICFCY）, function assessment, development, intervention, and functional influencing factor of children and adolescents with CP. The consistency of extracted data by 6 researchers was requested to be over 80%. Qualitative and quantitative methods were used to analyze data, and the core member of the CANDLE team answered the questions proposed by the scoping review. Main outcome measures：Questions proposed by the scoping review. Results：A total of 372 articles were enrolled in this scoping review. There has been a gradual increase in the number of guidelines, expert consensus, systematic reviews and scoping reviews in the past years. We got 7 English scoping reviews from 2010 to 2018, and 42 from 2018 to 2022. The majority of English literature focuses on intervention techniques, assessment techniques, and function status. Intervention techniques in Chinese literature accounted for 66.7%. There were 37 kinds of intervention techniques in 181 English articles. Four articles adopted ICFCY or ICF concept, and 78 articles partially used ICF concept in their structure. A total of 47 guidelines and expert consensus were enrolled. Among them, 9 expert consensus and 7 guidelines used function as a starting point. In 6 comprehensive guidelines, contents about function related to posture and motor were shown by muscular tension, mobility, hand activity, secondary musculoskeletal, daily living and leisure activities. There were 104 articles (31%) discussing the assessment method separately within 20 articles using COSMIN. Nine articles (3%) independently elaborated the effect of development characteristics on function. A total of 41 articles (12.1%) analyzed the interaction between function and background factors through the methods of correlation and influencing factors. Conclusion：The overall structure of CANDLE was initially formed through this scoping review. Eight domains were established with function as the core, each of which is shown through five elements (ICFCY as the framework, assessment, development, interaction, and intervention). Questions of interest within the scope are built on the elements.

Background：Authoritative clinical practice guidelines and expert consensus have recommended the use of rituximab (RTX) in the treatment of children with frequent relapsing/steroid-dependent nephrotic syndrome (FRNS/SDNS) of steroid-sensitive nephrotic syndrome(SSNS). However, there is a discrepancy in terms of clinical indications, treatment, and follow-up protocols. Objective：To comprehensively examine relapse and steroid therapy during over one year follow-up in patients diagnosed with FRNS/SDNS who were administered the initial course of RTX while being in remission. Design：Systematic review and meta-analysis. Methods：Keywords of SSNS, FRNS, SDNS, and RTX were used to search China Biology Medicine Database, PubMed, Embase, Scopus, and Cochrane from the inception to June 26, 2022. The preliminary and full-text screening were conducted by two independent reviewers, who also extracted the relevant data. Unsure or controversial literature underwent a third-party review. At least one RTX intervention for children aged between 1 and 22 years with SSNS was included. Main outcome measures：The frequency of relapse, time to the first relapse, cumulative steroid dosage, and percentage of patients who withdrew from steroids in those who were followed-up for more than 1 year after receiving RTX intervention. Results：A total of 26 studies (1 in Chinese and 25 in English) were eligible including 8 randomized controlled trials (RCT), 1 non-randomized study of interventions (NRSI), 8 cohort studies, and 9 case series reports. Two subgroups were defined as FRNS/SDNS+ group in which immunosuppressants were used before RTX intervention and FRNS/SDNS- group in which no other immunosuppressants were used before RTX intervention. Meta-analysis of 9 studies on the relapse rate of FRNS/SDNS during at least 1-year follow-up showed a 78% reduction in the relapse rate of RTX compared to the control group (OR=0.22, 95%CI: 0.09 to 0.53). In FRNS/SDNS+ group， RTX reduced the relapse rate by 67 % compared to the control group (OR=0.33, 95%CI: 0.12 to 0.94). In FRNS/SDNS- group, RTX reduced the relapse rate by 85 % compared to the control group (OR=0.15, 95%CI: 0.03 to 0.68) in the absence of other immunosuppressants. Additionally, the meta-analysis of 20 studies indicated that the relapse rate after RTX was 42% (95% CI: 32 to 53). The meta-analysis of 9 studies on the time to the first relapse of FRNS/SDNS+ during at least 1-year follow-up indicated that the median time to the first relapse was 9.89 months (95%CI: 7.14 to 12.65). According to the meta-analysis of 3 studies on the median relapse free survival (RFS) from the initiation of FRNS/SDNS- to at least 1-year follow-up, RTX (1-2 doses) had a longer median RFS of 20 days compared to the control, with a median survival ratio (MSR) of 0.69 (95%CI: 0.52 to 0.87). The meta-analysis of four studies on the reduction of FRNS/SDNS cumulative steroid dose over a 1-year period found that RTX produced a significant decrease compared to the control group with a statistically significant difference (SMD=-1.12, 95%CI: -1.49 to -0.74). According to the meta-analysis of two studies on FRNS/SDNS steroid withdrawal rate within a three-month follow-up, the rate of RTX was 14.6 times higher compared to the control group (CNI or CTX) (OR=14.62, 95% CI: 5.43 to 39.39). The meta-analysis of three studies on the steroid withdrawal rate of FRNS/SDNS+ during a six-month follow-up after RTX showed a withdrawal rate of 68% (95% CI: 56 to 79). Conclusions：Compared with controls, RTX showed limited benefits in terms of relapse rate and first relapse time from the initiation of treatment to 1-year follow-up, and it could provide benefits in steroid reduction rather than steroid withdrawal. Addition of RTX in FRNS/SDNS patients, as compared with placebo or blank control, can reduce the 1-year follow-up relapse rate by at least 88%. The overall relapse rate for FRNS/SDNS patients who received RTX treatment at the 1-year follow-up was 43%. The addition of RTX treatment for FRNS/SDNS can provide 10 months of relapse-free survival time.

Objective To explore the processing Methods of big data of hospital information systems(HIS) and analyze the characteristics and trends of outpatient visits and medical services in a tertiary pediatric hospital in Shanghai during the past 10 years. Methods Data were extracted from the database of outpatient registry in HIS from 2009 to 2018. Data cleaning (diagnosis, address, replication, outliers, and missing data), data assembly (linkage of database), data reduction, data anonymization and data audit were conducted according to the procedure of big data. We analyzed the characteristics and trends of outpatient visits and medical services. Results The total outpatient visits from 2009 to 2018 were 20,775,899 and 18,242,822 visits were included in this study. Males were 4,656,843 accounting for 57.42%. Patients under 1 year old, from 1 to 3 years old, from 3 to 7 years old and from 7 to 18 years accounted for 9.57%,40.94%, 24.82% and 24.67% respectively. The missing data rate of diagnosis, address, and name of doctors was 5.92%, 45.31% and 28.61%,respectively. There were no significant differences in the percentage of sex and age in different years among the population with missing data. The average increasing rate of outpatient visits was 5.22% in the past 10 years. The increasing rate of 2012 and 2014 were 11.22% and 10.93% respectively, compared with the same period of last year. The increasing rate slowed down to 2.90% after 2015. The distribution of outpatient address showed the trends of expansion from the east of China to the South-west, North-west, South, Middle and North-east. The average workload of a doctor was 51 visits in a day. According to professional titles, there were 63, 54, and 45 visits per day for junior, intermediate, and senior doctors, respectively. The number of specialty clinics increased from 46 in 2009 to 180 in 2018 and serviced the 68.12% of outpatient visits. The number of specific disease clinics increased from 16 in 2009 to 142 in 2018. Conclusion The big data of outpatient visits in a tertiary pediatric hospital(National Children's Medical Center) in China showed that there was a sustainable upward trend in outpatient visits; the origin of patients expanded from Shanghai to the whole of China; the average visits for a single physician were lower than that of the country. Specialized clinics in specialties or diseases are an important trends for the development of children's hospitals.

Objective To assess the validation of Wong-Baker FACES Rating Scale（Wong-Baker）in confort assessment among children with acute fever and aged 0-5 years about comfort and corresponding relationship between Wong-Baker and Echelle Douleur Inconfort Nouveau-Ne, neonatal pain and discomfort scale（EDIN）. Methods This was a prospective，observational study of children aged from 0 to 5 years with acute fever about comfort. Children's discomfort severity with fever was rated on EDIN and Wong-Baker. Spearman's correlation (r) was used to measure agreement between two scales. Results A total of 97 patients including 56 males were enrolled with 31 cases aged ≤1 year, 49 aged -3 years, 17 aged -5 years and 35 cases ≤38.0℃, 49 with -39.0℃ and 13 ≥39.0℃. Forty three cases (44.3%) were diagnosed as infectious diseases, 25 (25.8%) with respiratory diseases, 21 (21.6%) with fever of unknown causes, 7 with hematology diseases and 1 with digestive disease. All children with fever felt pain assessed by Wong-Baker scale, 29.9% of them felt mild pain. The assessments of obvious pain, severe pain and fierce pain were accordant between genders and age groups. The feeling to different pain became more obvious with the increasing of body temperature. More than 90% children felt uncomfortable assessed by Chinese version EDIN scale. It was accordance with the feeling scored 1 to 3 between different genders and ages. The number of children with good sleep, friendly with study staff and easily pacify decreased with increasing of body temperature. Agreement between Wong-Baker and EDIN was good（r=0.742, P<0.001) without relationship with gender, age or body temperature. Conclusion Both scales could be used to assess comfort in acute fever children aged from 0 to 5 years with good agreement between Wong-Baker and EDIN.

Background Sitting height/leg length ratio (SH/LL) and Sitting height/height ratio (SH/H) are the important indicators of body proportion assessment. Their scientific assessment will supply valuable information in clinical practices, but the detailed data on their growth reference standards have not been reported. Objective To construct the SH/LL and SH/H growth charts for Chinese children and adolescents from birth to 18 years. Design A cross-sectional survey. Methods Stature and sitting height of 92 494 healthy urban children aged 0-18 years were measured in two national surveys in 2005 in China, which named The National Survey on the Physical Growth and Development of Children in the Nine Cities of China and The Chinese National Survey on Student's Constitution and Health. Leg length was calculated by height minus sitting height and the SH/LL ratio and SH/H ratio were calculated. Growth reference standards of SH/LL and SH/H were constructed using the LMS method. Main outcome measures Reference values of SH/LL and SH/H. Results The Z-scores growth charts of SH/LL and SH/H (-3SD, -2SD, -1SD, Median, +1SD, +2SD, +3SD) were constructed. The median of SH/LL declined from birth (2.00) to 13 years in boys (111) and to 11 years in girls (1.13), then increased slightly to the age of 18 (1.16 in boys and 1.18 in girls). A similar growth pattern was found in the SH/H, that is, the median of SH/H was 0.67 at birth and declined to the lowest point (0.53) with age, then increased slightly to 0.54 at 18 years old. Conclusions The growth charts of SH/LL and SH/H are used in assessing body proportions for Chinese children and adolescent individuals, and they could be used in conjunction with the growth charts of height, weight for Chinese children aged 0-18 years.

Background：The 2019 international consensus on central precocious puberty (CPP) proposed that random serum luteinizing hormone (LH) has important reference value for the confirmation or exclusion of CPP, but there is no corresponding diagnostic criteria in China. Objective：By verifying the diagnostic value of the cutoff value of serum LH basal value proposed in the international consensus in 2019 for CPP, it is expected to reduce unnecessary GnRH stimulation tests and provide guidance for clinical practice. Design：Diagnostic accuracy study. Methods：According to the Chinese Expert Consensus on the Diagnosis and Treatment of Central Precocious Puberty (2022 edition) (referred to as the Chinese consensus), the recommended value of LH (chemiluminescence method) (peak LH ≥5 IU·L-1and peak LH /FSH ≥0.6 combined with clinical) was used as the gold standard for the diagnosis of CPP. According to the two cutoff values (0.83 and 0.20 IU·L-1) recommended by the international consensus, girls aged 49 years who met the diagnostic criteria of precocity, did not have menarche, had breast development Tanner stage Ⅲ or below, and underwent GnRH stimulation test were enrolled. Peripheral precocity was excluded. The clinical data of age, height, weight, body mass index (BMI), Tanner stage, gonadal axis hormones and sex hormones, bone age (TW3), pituitary MR and uterine and ovarian ultrasound were collected. Main outcome measures：Sensitivity and specificity of different basic luteinizing hormone cutoff values for diagnosing CPP. Results：A total of 352 girls with precocious puberty were included in the analysis. Among them, 203 cases were diagnosed as CPP and 149 cases were diagnosed as PT according to the gold standard. The average age of precocious puberty was (7.3±1.0) years, and the average height was (130.6±10.3) cm. The CPP girls had significantly higher mean age, height and bone age, and baseline serum LH, FSH, LH/FSH, and E2 levels than the PT girls. The differences were statistically significant. The specificity of baseline serum LH value ≥0.83 IU·L-1 (100%, 95%CI:97%100%) was better than that of baseline serum LH value <0.20 IU·L-1(45%, 95%CI:37%53%) and from 0.20 to 0.82 IU·L-1 (56%, 95%CI:47%63%). The CPP girls with a baseline LH level of ≥0.83 IU·L-1 had significantly higher breast Tanner stage, bone age, and bone agechronological age difference than those with a baseline LH level of <0.83 IU·L-1 (P<0.05), and a significantly lower pituitary height than those with a baseline LH level of < 0.83 IU·L-1 (P<0.05). Conclusions：When chemiluminescence method is used to detect gonadal hormone in children with precocious puberty, CPP can be diagnosed with serum LH base value ≥ 0.83 IU·L-1, and GnRH excitation test is not required LH <0.20 IU·L-1 cannot be used as the exclusion criteria for CPP, and sexual GnRH excitation test should be selected to assist diagnosis based on clinical characteristics.

Objective：To determine the current status of pubertal development and prevalence of sexual precocity among Beijing school-aged boys. Methods：As part of the Beijing Child and Adolescent Metabolic Syndrome Study (BCAMS), a cross-sectional study on the presence of secondary sexual characteristics among 9 815 school-aged boys aged 6~18 years was undertaken in Beijing city from April to October 2004. All participants completed a questionnaire on demographic variables. The stages of genital and pubic hair development were assessed through visual inspection using standardized drawings and descriptions based on Tanner criteria. The larger-sided testicular size was measured using Prader orchidometer and recorded in milliliters. Age at spermarche was collected by self-report. Status quo method and probit transformation analysis were used to calculate the median age at spermarche and at onset of pubic hair (P2), gentile (G2) and the age at testicular volume34ml. Sexual precocity was defined as onset of P2 or one-sided testicular volume 3 4 ml before 9 years of age. Results：Data were analyzed for 9 815 boys, of whom 50.5% resided in urban and 49.5 % in rural. Spermarche was at age of (14.4±1.2）years in all boys,（14.1±1.1）years in urban boys and (14.8±1.2) years in rural boys. The median age of P2 was (12.2±1.2) years for all, (12.0±1.2) years for urban boys and (12.4±1.2) years for rural boys. Testicular development (34ml) was observed at age of （10.7±1.2) years for all,（10.8±1.2) years for urban boys and (10.7±1.2)years for rural boys. The prevalence rates of sexual precocity according to pubic hair and testicular size were 1.92% and 0.17%, respectively. Conclusions：Difference of pubertal development staging between urban boys and rural boys was observed in this study. More studies on pubertal development in boys are necessary.

Background：The severity of mycoplasma pneumoniae pneumonia (MPP) is related to the formation of mucus plugs (MUP) in the airway, but the current MUP detection mainly depends on bronchoscopy. Objective：To establish a nomogram predictive model based on clinical features and plasma cytokines for airway MUP formation in MPP children. Design：Case -control study. Methods：Children who were diagnosed as MPP and underwent bronchoscopy were classified into MUP and non -MUP groups. Clinical features, inflammatory markers and cytokines in both plasma and BALF were collected. Univariate analysis was performed to identify statistically significant clinical features, inflammatory markers, and cytokine risk factors between the two groups. Spearman correlation analysis was conducted to assess the correlation between inflammatory markers, plasma cytokines and BALF cytokines and to exclude collinearity issues. A receiver operating characteristic (ROC) curve was generated to assess the predictive performance of a multivariable logistic regressionbased predictive model using the R package of RMS. Main outcome measures：Predictive efficacy of the nomogram model for predicting the probability of airway mucus plug formation in children with MPP. Results：A total of 263 children with MPP were included in the analysis, including 134 males and 129 females, with an average age of (7.0 ± 0.2) years. There were 82 (31.2%) cases in the MUP group and 181 cases in the non -MUP group. Univariate analysis showed significant differences between the two groups in age, maximum temperature before bronchoscopy, decreased breath sounds upon auscultation at admission, chest CT showing pulmonary consolidation, imaging suggesting pleural effusion, extrapulmonary complications, proportion of severe pneumonia, neutrophil percentage, CRP, PCT, D -dimer, LDH, IgA, lymphocyte percentage, and ALT. Cytokine analysis revealed significant differences between the two groups in levels of IL -5, IL -6, IL -8, and IFN -γ in plasma, as well as in levels of IL -1β, IL -5, IL -8, IL -10, IFN -γ, and TNF -α in BALF. Spearman correlation analysis showed there was no collinearity in neutrophil percentage, CRP, PCT, LDH, D -dimer, IgA, plasma IFN -γ, plasma IL -6, plasma IL -5, and plasma IL -8; there was a certain correlation between blood inflammatory markers and BALF cytokines; plasma cytokines (IFN -γ, IL -6, IL -5, and IL -8) were positively correlated with their corresponding BALF cytokines. The nomogram predictive model for the formation of airway mucus plugs in MPP patients, based on age, pleural effusion, D -dimer, and plasma IFN -γ levels, had an AUC of 0.817 (95% CI: 0.747 -0.889) with the sensitivity of 79.0% and specificity of 69.1%. Conclusion：The nomogram predictive model based on age, pleural effusion, D -dimer, and plasma IFN -γ levels had a good predictive performance for the formation of airway mucus plugs in MPP patients.

Background：The Swanson, Nolan, and Pelham, version scale (SNAP-Ⅳ) is the most widely used assessment tool for evaluating the core symptoms of attention-deficit/hyperactivity disorder (ADHD) in children. However, there is still a lack of systematic review of the measurement properties of the SNAP-Ⅳ scale, which may introduce bias and potentially affect the assessment validity of the measurement tool. Objective：To evaluate the measurement properties of the SNAP-Ⅳ based on the Consensus-based Standards for the selection of health Measurement Instruments (COSMIN), and to provide evidence-based measurement instrument recommendations for the screening, diagnosis and efficacy evaluation of children with ADHD. Design：Systematic review. Methods：Related literature was systematically searched in databases of China National Knowledge Infrastructure (CNKI), Wanfang Database, VIP Database, China Biomedical Literature Database (CBM), PubMed, Cochrane Library, EMBASE, and Web of Science from the inception to April 21, 2022. Two reviewers independently screened the literature, and the measurement properties of the SNAP-Ⅳ scale were objectively evaluated according to the COSMIN manual. Main outcome measures：Content validity and internal consistency of the SNAP-Ⅳ scale. Results：A total of 14 studies involving 6 language versions were included. The content validity of the SNAP-Ⅳ scale was rated as "sufficient" (moderate quality evidence) in terms of relevance, comprehensiveness, and comprehensibility. However, there was no reported content on the development of the SNAP-Ⅳ scale. The parent and teacher versions of the SNAP-Ⅳ scale showed "sufficient" internal consistency (high-quality evidence). There was no high-quality evidence indicating "insufficient" measurement properties. The overall recommendation was classified as level A. Conclusion：The SNAP-Ⅳ scale is recommended for the screening, diagnosis, and treatment evaluation of children with ADHD, and its results can be considered reliable.

Background: The high prevalence of hearing loss in China is associated with a high carrier rate of pathogenic variants in genes for hearing loss. At present, there is a lack of screening data on carriers of pathogenic genes for hearing loss among newborns in neonatal intensive care units (NICU). Objective: To investigate the carrier rate of GJB2 and SLC26A4, pathogenic genes for hearing loss, in NICU newborns. Design: Cross-sectional survey. Methods: Newborns were enrolled in the NICU at Children's Hospital of Fudan University from January 2016 to December 2021, with admission days≤28 d and high-throughput sequencing completed before discharge. Those with positive hearing-loss-associated genetic findings after birth were excluded. The sex, gestational age and birth weight were collected from electronic medical records. The number of cases identified with pathogenic variants in GJB2 and SLC26A4 and the standard terminology of the human phenotype of the children were extracted from the sequencing database. Carrier rate(%)=the number of heterozygous pathogenic or likely pathogenic (P/LP) variants/total number of study subjects. PubMed, Embase, and Wanfang databases were searched. Literature that previously reported the carrier status of the GJB2 gene and/or SLC26A4 gene P/LP mutations in NICU patients, newborns and pregnant women in China were included and reviewed. Main outcome measures: Carrier rate of P/LP variants in the GJB2 gene and SLC26A4 gene. Results: A total of 14 924 neonates were included, including 8 587 males (57.5%) and 6 337 females, with a gestational age of (35.6±3.7) weeks and a birth weight of (2 711.7±887.1) g. A total of 18 heterozygous P/LP mutations were detected in 2 009 children (13.462%) carrying P/LP mutations of the GJB2 gene, among which c.109G>A was the most common (10.902%), followed by c.235del (1.749 %), c.299_300del (0.409%), c.176_191del (0.154%), c.508_511dup (0.074%) and c.257C>G. There were 305 children (2.044%) carrying P/LP mutations of the SLC26A4 gene. A total of 31 heterozygous P/LP mutations were detected, and the six highest carrier rates were for c.919-2A>G (1.139%), c. .2168A>G (0.181%), c.1226G>A (0.100%), c.1229C>T (0.094%), c.1174A>T (0.080%) and c.1003T>C, respectively. Conclusions: It is suggested that c.109G>A, c.508_511dup and c.257C>G on the GJB2 gene and c.1003T>C on the SLC26A4 gene should be included in the carrier screening for pathogenic genes for hearing loss among newborns in NICU.