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中国循证儿科杂志

中国循证儿科杂志 ›› 2016, Vol. 11 ›› Issue (6): 460-462.

• 论著 • 上一篇    下一篇

克罗恩病和健康儿童血清细胞因子水平的病例对照研究

卢华君，赵忠艳，李小莉，闫秀梅，林小春，黄开宇   

  1. 1 温州医科大学附属第二医院 温州，325027；2 育英儿童医院儿童消化科 温州，325027
  • 收稿日期:2016-10-27 修回日期:2016-12-16 出版日期:2016-12-25 发布日期:2016-12-25
  • 通讯作者: 黄开宇

A case-control study of serum levels of cytokines in Crohn's disease and healthy children

LU Hua-jun, ZHAO Zhong-yan, Li Xiao-li, YAN Xiu-mei, LIN Xiao-chun, Huang Kai-yu   

  1. Department of Pediatric Gastroenterology, Yuying Children's Hospital, Wenzhou 325027, China; The Second Affiliated Hospital of Wenzhou Medical University, Wenzhou 325027, China
  • Received:2016-10-27 Revised:2016-12-16 Online:2016-12-25 Published:2016-12-25
  • Contact: HUANG Kai-yu

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摘要：

目的 观察克罗恩病（CD）患儿血清细胞因子水平，探讨细胞因子水平紊乱在儿童克罗恩病发病中的作用。 方法 纳入2013年1月至2015年12月在温州医科大学育英儿童医院（我院）儿内科住院的确诊CD患儿（病例组，根据CD活动度分为不同亚组）和同期在我院门诊体检的健康儿童，采用流式分析技术检测晨起空腹静脉血血清中IL-2、IL-4、IL-6、IL-10、TNF-α、IFN-γ和IL-17A的水平。 结果 病例组32例，男18例，女14例，年龄（8.5±4.3）岁，其中CD轻度活动亚组12例，CD中重度活动亚组20例；对照组30名，男18名，女12名，年龄（10±3.5）岁。病例组和对照组年龄、性别比较差异无统计学意义（P>0.05）。病例组患儿血清IL-2、IL-4、IL-6、TNF-α和IL-17A水平高于对照组，血清IL-10和IFN-γ水平低于对照组，差异均有统计学意义（P＜0.05）。此外，根据儿童CD活动指数（PCDAI）评分为中重度活动亚组患儿的血清IL-2、TNF-α和IL-17A水平高于轻度活动亚组患儿，差异有统计学意义（P＜0.05）。 结论 与健康儿童相比，CD患儿存在明显的免疫相关细胞因子水平变化，而且疾病活动度不同其细胞因子水平亦有差异，提示细胞因子水平紊乱在儿童CD发病及病情的发展中有重要作用。

Abstract:

Objective To detect the serum levels of cytokines in children with Crohn's disease (CD), in order to explore the role of cytokine levels disorder on the pathogenesis of CD in children. Methods Hospitalized CD patients diagnosed between January 2013 and December 2015 in Yuying Children's Hospital of Wenzhou Medical University （Case group was divided into different subgroups according to CD activity），and outpatient healthy children at the same period were enrolled as case group and control group, respectively. The levels of serum IL-2, IL-4, IL-6, IL-10, TNF-α, IFN-γ, and IL-17A were detected by flow cytometry in the morning with an empty stomach. Results There were 32 cases of children with CD in case group, including 18 males, 14 females, with average age of (8.5±4.3) years; 12 cases in mild activity CD subgroup, 20 cases in moderate and severe activities CD subgroup.There were 30 healthy children in control group, including 18 males and 12 females , with average age of (10±3.5) years. There was no statistically significant difference in age and gender between case group and control group(P>005).The levels of serum IL-2, IL-4, IL-6, TNF-α, and IL-17A in case group were significantly higher than those in the control group (P＜0.05). And the levels of serum IL-10 and IFN-γ in case group were significantly lower than those in the control group (P＜0.05). In addition，the levels of serum TNF-α, IL-2 and IL-17A in moderate and severe activities CD subgroup identified by the pediatric CD activity index (PCDAI) were significantly higher than those in mild activity CD subgroup (P＜0.05). Conclusion Compared with healthy children, CD children existed obvious immune related cytokines level change.With the change of CD activity, the levels of cytokines were also different. It suggested that cytokine levels disorder played an important role in the development of CD in children.