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中国循证儿科杂志

中国循证儿科杂志 ›› 2023, Vol. 18 ›› Issue (6): 452-455.DOI: 10.3969/j.issn.1673-5501.2023.06.008

• 论著 • 上一篇    下一篇

基于高通量测序对中国南北方地区学龄前健康儿童肠道菌群的横断面调查

董文迪1,金霞霞2,莫珊1,3,姜美琳1,刘艳欢1   

  1. 1  广州中医药大学佛山临床医学院 佛山，528000；2  中国中医科学院中药资源中心道地药材品质保障与资源持续利用全国重点实验室 北京，100700；3佛山复星禅诚医院 佛山，528000
  • 收稿日期:2023-10-24 修回日期:2023-11-28 出版日期:2023-12-25 发布日期:2024-01-22
  • 通讯作者: 莫珊

Intestinal microflora diversity in healthy preschool child aged 3-7 in northern and southern China based on high-throughput sequencing: A cross-sectional survey

DONG Wendi1,  JIN Xiaxia2, MO Shan1, 3,JIANG Meilin1, LIU Yanhuan1   

  1. 1 Foshan Clinical Medical College, Guangzhou University of Chinese Medicine, Foshan 528000, China; 2 Chinese Medicine Resource Center, China Academy of Chinese Medical Sciences, Beijing 100700, China; 3 Foshan Fuxing Chancheng Hospital, Foshan 528000, China
  • Received:2023-10-24 Revised:2023-11-28 Online:2023-12-25 Published:2024-01-22
  • Contact: MO Shan

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摘要： 背景：肠道微生物群可通过多种途径影响人类的健康并与疾病的发生有关，不同地区人群中的肠道微生物构成差异明显。 目的：通过16S rRNA基因测序方法比较中国南北方地区学龄前健康儿童肠道微生物构成的差异。 设计：横断面调查。 方法：选择 3~7岁学龄前健康儿童，南方组为广东省佛山市佛山复星禅诚医院体检中心体检儿童，北方组为新疆维吾尔自治区塔城地区某幼儿园在园儿童。由经过培训的家长采集儿童的粪便样本，由杭州谷禾信息技术有限公司完成肠道菌群检测及相关数据分析。 主要结局指标：菌群多样性，物种组成结构差异。 结果：2023年2月1日至2023年4月1日南方组纳入45名，年龄（4.0±1.5）岁；北方组纳入43名，年龄（4.1±1.3）岁；两组的性别构成、年龄、民族构成差异均无统计学意义。北方组Shannon、Simpson、Chao、ACE指数均高于南方组，差异均有统计学意义。PCoA图显示加权后观察到两组样本显著分离。北方组厚壁菌门的丰度高于南方组（55.1% vs 39.2%）、南方组变形菌门的丰度高于北方组（18.6% vs 7.2%）、南方组大肠埃氏菌属-志贺氏菌属的丰度高于北方组（11.8% vs 2.2%）、北方组粪杆菌属的丰度高于南方组（13.9% vs 6.3%）,差异均有统计学意义。通过LEfSe分析及随机森林组间预测，在南方组找到大肠埃氏菌属-志贺氏菌属和活泼瘤胃球菌属2个差异有统计学意义的生物标志物，北方组找到粪杆菌属、琼脂杆菌属、毛螺菌属、塞内加尔厌氧菌属、罕见小球菌属、挑剔真杆菌属、副萨特氏菌属、小类杆菌属、罗氏菌属9个差异有统计学意义的生物标志物。 结论：中国南北方学龄前儿童肠道菌群存在显著差异，在未来进行肠道菌群与疾病的研究时，须充分考虑宿主的地域性。

关键词: 肠道菌群, 地域, 学龄前儿童

Abstract: Background: Intestinal microbiota can affect human health through various ways and is related to the occurrence of diseases. There are obvious differences in the composition of intestinal microbiota in different regions. Objective: To compare the difference of intestinal microbial composition among healthy preschool child in northern and southern China by 16S rRNA gene sequencing method. Methods: Children aged 3-7 years who underwent physical examination at the physical examination center of Foshan Chancheng Hospital in Foshan City, Guangdong Province were selected as the southern group, and children from a kindergarten in Tacheng District of Xinjiang Uygur Autonomous Region were selected as the northern group. Stool samples of all preschool children were collected by their parents after training, and intestinal flora detection and related data analysis were completed by Hangzhou Guhe Information Technology Co., LTD. Main outcome measures: Microbial diversity, as well as differences in species composition. Results: From February 1, 2023 to April 1, 2023, 45 children aged (4.0±1.5) years were included in the southern group. In the northern group, 45 cases were included, of which 2 were unqualified, and the remaining 43 were (4.1±1.3) years old. There were no significant differences in gender, age and ethnic composition between the two groups. The Alpha diversity indexes including Shannon, Simpson, Chao and ACE of the northern group were higher than those of the southern group with statistically significant differences between two groups. The weighted unifrac PCoA plot showed a significant separation between the two group samples. The abundance of Firmicutes in the northern group was higher than that in the southern group (55.1% vs 39.2%), and the abundance of Proteobacteria in the southern group was higher than that in the northern group (18.6% vs 7.2%). The abundance of Eubacterium-eligens-group in southern group was higher than that in northern group (11.8% vs 2.2%), and the abundance of Faecalibacterium in the northern group was higher than that in the southern group (13.9% vs 6.3%). The differences were statistically significant. LEfSe analysis and random forest intergroup prediction revealed that statistically different biomarkers were Escherichia-Shigella and Ruminococcus-gnavus group for the southern group and Faecalibacterium, Agathobacter, Lachnospira, Senegalimassilia, Subdoligranulum, Eubacterium-eligens-group,Parasutterella, Dialiste and Rseburia for the northern group. Conclusion: There are significant differences in the intestinal flora of preschool children in the north and south of China. The regionality of the host should be fully considered in the future research on intestinal flora and diseases.

Key words: Intestinal microbes, Region, Preschool child