基于GAMLSS方法构建重庆地区6～12岁儿童 血清维生素A参考值的横断面调查

摘要/Abstract

摘要： 目的构建重庆地区6～12岁儿童血清维生素A（VA）水平参考值。方法采取分层整群抽样的方法选取重庆地区1个主城区和1个县，各随机抽取2个街道，街道内的所有小学根据年级分组抽取班级，同时取得家长和儿童知情同意后采集静脉血，用高效液相色谱仪检测VA。基于位置、尺度、形状广义的可加模型（GAMLSS）绘制性别别、年龄别血清VA水平的P1、P5、P25、P50、P75、P95、P99百分位数曲线图。结果重庆市九龙坡区和丰都县各2个街道的6所小学的36个年级的1 110名健康儿童采集了外周静脉血，最终1 077例健康儿童纳入本研究，平均年龄（9.52±1.63）岁，男570人（529%），城市480人（44.6%），6～12岁各年龄组的儿童样本数分别为62、105、92、198、279、257、84人。总体、男童和女童的GAMLSS结果中，VA服从Box-Cox-Cole-Green（BCCG）分布，理论百分位数和样本的百分位数几乎落在同一直线上，残差分布均匀。6～12岁男童与女童血清VA百分位数曲线存在交叉，男童血清VA随年龄呈现持续缓慢上升趋势，女童血清VA水平随年龄的增长基本维持不变。结论本研究构建了重庆地区6～12岁儿童血清VA的百分位曲线图，发现男女童血清VA随年龄变化的趋势不一致。

Abstract: Objective To explore the reference value of serum Vitamin A (VA) of children aged 6 to 12 years in Chongqing, China. Methods In this study, a stratified cluster sampling method was adopted to randomly select two streets respectively in one urban district and one county in Chongqing, and classes of all primary schools in the street were sampled according to different grades. Blood samples of children were collected after the parents and children signed informed consents. High Performance Liquid Chromatography was used to detect serum VA. The Generalized Additive Model for Location, Scale and Shape (GAMLSS) was used to plot the P1, P5, P25, P50, P75, P95 and P99 percentile curves of serum VA by gender and age. Results Peripheral venous blood was collected from 1,110 healthy children in 36 grades of 6 primary schools in 2 streets of Jiulongpo District and 2 streets of Fengdu County in Chongqing and 1，077 healthy children were finally included in this study. The mean age of the participants was 9.52±163 years, including 570(52.9%) boys and 480(44.6%) urban children. The sample size of children from 6 to 12 years old were 62, 105, 92, 198,279, 257 and 84, respectively. GAMLSS results of overall, boys, girls indicated that VA conformed to Box-Cox-Cole-Green (BCCG) distribution, the theoretical percentile and the sample percentile almost lay on the same straight line, and the residuals were evenly distributed. The percentile curves of serum VA between boys and girls aged 6 to 12 years were crossed. In boys, the serum VA increased slowly with age, while the serum VA remained unchanged with age in girls. Conclusion This study established the percentile curve of serum VA of children aged 6 to 12 years in Chongqing. The trend of serum VA with age was disparate between boys and girls.

瞿平罗业涛梁小华王旭芹刘友学李廷玉陈洁杨亭. 基于GAMLSS方法构建重庆地区6～12岁儿童血清维生素A参考值的横断面调查[J]. 中国循证儿科杂志, 2020, 15(2): 135-139.

QU Ping, LUO Ye-tao, LIANG Xiao-hua, WANG Xu-qin, LIU You-xue, LI Ting-yu, CHEN Jie, YANG Ting. Research on the reference value of serum vitamin A of children aged 6 to 12 years in Chongqing by GAMLSS: A cross-sectional survey[J]. Chinese Journal of Evidence -Based Pediatric, 2020, 15(2): 135-139.